A Dorchester man, convicted in 2009 for illegal gun possession, is facing new gun possession charges after a housekeeper allegedly found a loaded gun and more than a quarter-pound of marijuana in his South Boston hotel room, Suffolk County prosecutors said.

Reginald Weaks was charged with unlawful possession of a firearm as a subsequent offense, unlawful possession of ammunition, improper storage of a firearm, and possession with intent to distribute marijuana during his arraignment Tuesday in South Boston Municipal Court, according to Suffolk District Attorney Daniel F. Conley’s office.

Weaks, 33, pleaded not guilty to all charges.

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Believing Weaks had checked out, a housekeeper entered his Holiday Inn Express room on March 4. She found a gray and black Nike backpack next to the bed, Boston police said.

When the woman picked up the backpack to bring it to lost and found, she allegedly saw four plastic bags filled with marijuana, and a silver firearm inside, police said. She also found a “nugget of a green leafy substance” in an open drawer in the room. She alerted her supervisor, who secured the room until police arrived.

When officers arrived at the Boston Street hotel, they found 4.8 ounces of marijuana, and a Raven Arms MP-25 semi-automatic pistol with live rounds of .25-caliber ammunition in its magazine, police said.

Officers checked hotel records and identified Weaks as the person who had rented the room, but police also learned that the housekeeper was mistaken and Weaks had extended his stay for another night, which is why the bag was still in the room.

Police left, but told employees to notify them if Weaks returned, police said.

When Weaks returned later that day, employees told him that police had taken possession of his bag and officers were on the way. Weaks fled. He was eventually tracked down and arrested by police in Dorchester Tuesday, prosecutors said.

In court, Judge Jonathan Tynes set bail at $50,000 cash and ordered Weaks to wear a GPS monitoring device, abide by a curfew, and stay away from the location of the incident in the event he is released on bail.

Weaks previously served two years in jail for a 2009 gun conviction in Suffolk Superior Court and was previously convicted of possessing Class B and Class D substances with intent to distribute, prosecutors said.

He is to return to court March 31, prosecutors said.